You will need access to water for this activity.

We suggest students work in pairs and two pairs of students share supplies at the same table group.

Prepare the Purple Indicator Liquid

Put 1 cup of black beans into 2 cups of water and leave them for at least an hour. The beans will soak up some water and turn the rest purplish brown. Drain the beans and reserve the purplish-brown liquid.

Prepare the Straws

Cut each straw in half to make two short straws. Students will use these to transfer drops of liquid. (Full-length straws are likely to tip over cups.)

Prepare the Testing & Acid Reaction Supplies

Gather all of your Dixie cups and separate them into seven equal piles. You will fill the cups in each of these piles with a different liquid or powder.

• Water Cups: Add 2 tablespoons of water.
• Vinegar Cups: Use a permanent marker to label each of these cups with a “V” so students can quickly tell these apart from the water. Add 2 tablespoons of vinegar.
• Baking Soda Cups: Add 2 tablespoons of baking soda. (You may want to tell students that baking soda is different from baking powder.)
• Baking Powder Cups: Use a permanent marker to label each of these cups with a “BP” so students can quickly tell these apart from the baking soda. Add 1 tablespoon of baking powder.
• Purple Indicator Cups: Add 1 tablespoon of the purple indicator liquid that you prepared.
• Acid Substance “A” Cups: Add 1 tablespoon of mustard.
• Non-acid Substance “B” Cups: Add 1 tablespoon of a salt water substance. To make salt water, mix 1 tablespoon of salt into a cup of water.

Separate Supplies for Easy Distribution

We recommend you set up four supply stations.

Station A: Acid Reaction Supplies

• “Acid Reaction Supplies” printouts
• Water Cups
• Vinegar Cups
• Straws (2 per group)

From this station, each group of students will bring the following back to their desk:

Station B: Testing Supplies

• “Testing Supplies” printouts
• Baking Soda Cups
• Baking Powder Cups
• Purple Indicator Cups
• Stir Sticks (2 per group)
• Straw (1 per group)

From this station, each group of students will bring the following back to their desk:

Station C: Worksheets & Clean-up Supplies

• "Mixing Sheet" printouts
• "Results" printouts
• Sheet Protector
• Paper Towels

From this station, each pair of students will bring the following back to their desk:

Station D: Substances

• “A” Cups (mustard)
• “B” Cups (salt water)
• Straw for salt water (1 per group)
• Stir Stick for mustard (1 per group)
• Toothpicks

From this station, each pair of students will bring the following back to their desk:

Teacher Background

The purple liquid that you prepare from the black beans is called an indicator. There’s a pigment in black beans that changes color when it reacts with an acid or base. You and your students should notice that the color of the liquid changes to a reddish/pink when you add it to any of the acids (e.g. vinegar). You can then use this information to test unknown liquids. If the liquid turns pink, then it’s an acid.

You and your students will also notice that when baking soda is mixed with vinegar, there is fizzing that indicates an acid-base reaction. But baking soda does not fizz when mixed with water, making it a good acid indicator. Baking powder will also fizz with vinegar. But you will notice that baking powder will also slightly fizz when water is added. This is because baking powder is actually a mixture of baking soda (base) and cream of tartar (acid). This is why it reacts with both water and vinegar. So baking powder is not a good indicator because it fizzes when any liquid is added.